What are the primary defects associated with welding druring construction-stages which might influence the capacity of the gusset-plate connections??

Dear researchers What are the primary defects associated with welding druring construction-stages which might influence the capacity of the gusset-plate connections in concentrated braced frames?...

06 January 2021 797 3 View

Why T98g cells were dead after plamid transfection?

I constructed a plasmid, and extracted more than 500ug plasmid. I transfected it with four different reagent treatments, including two kind of nanoparticles ,pei and lipofectamin 3000. as a sample...

30 December 2020 7,412 0 View

Does anyone here uses the Software for Roche LightCycler 2.0 instrument?

Hi everyone, If anyone here uses Roche LightCycler 2.0 instrument for qPCR experiments, I would appreciate if they message me. I have some questions about it. Best - Navid

12 October 2020 6,234 2 View

What is the best equipment to find the thermal conductivity of a heatsink?

Dear All, I am trying to find the cooling efficiency of my heatsink and I am wondering if there is any equipment to do this thermal test for me. I am looking to find equipment to find a point to...

30 August 2020 3,961 8 View

What would be the "Natural Resource Management" in PAST-covid19 era?

In your opinion, what will be the attitude of human to the natural resources management (environment, water resources, soil resources, etc.) after the COVID-19 era?

18 May 2020 210 16 View

I am interested to know how can I modeling Troposphere with Bernese software? It means that can you introduce a source for it?

I readed the Bernese manual which that published at Bernese website, but it was'nt enough.

10 March 2020 6,098 3 View

Would you share me your opinions about mechanized harvesting of onion?

Onion harvesting is not mechanized in Iran. I am searching for the best harvesting machines of onion. Would you share me your opinions?

15 July 2019 3,649 6 View

How to percipitate Extracellular Vesicules (EVs) from body fluids using Polymer Based approach???

It would be great if someone gives me a detailed protocol for precipitation of exosome by non-PEG based and PEG based methods from body fluids???

05 May 2019 8,151 1 View

How to extract shape file of HRUs created by SWAT?

25 February 2019 3,822 2 View

PHREEQC based simulation for precipitation of salts in Ca-Mg-Na-K/Cl-H2O system during evaporation?

I want to predict the salt precipitation in Ca-Mg-Na-K/Cl-H2O system. In fact, I have a carnallite sample (containing 20 g CaCl2, 270 g NaCl, 180 g KCl and 140 g NaCl) and after complete...

09 January 2019 1,084 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View