In SDS PAGE.,before staining procedure to be carried out, which one among the solution will be best for protein fixation?
(low molecular weight 2kDa to 12kDa)
50% MeOH 50% H2O
25% Acetic acid or in TCA?
In tricine SDS PAGE the fixing solution used is 50% Methanol 10% acetic acid with 100 mM ammonium acetate for peptides as low size as 1 kDa. The gel composition is same as the normal SDS PAGE. so i think this would be the most suitable fixative.
Source: http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.4.html?foxtrotcallback=true
Agreed I have always used 50% methanol 10% acetic acid. The key to high and low molecular weight proteins is Tricine gels with MES running buffer
what if used, tris HCl and glycine/tris buffer()Running buffer) ? will it not work?
- Badges
- Science method