If their is no fragmentation of DNA in cancerous cell lines, does it proves their has been no apoptosis?

Where as in case of MTT assay, the results were positive, but in DNA fragmentation assay, their is no fragmentation of genomic DNA can be seen. So, my question is, is the DNA fragmentation solely...

07 August 2019 8,058 7 View

SDS Page - 10, 12, and 15%. Which will give better resolution for low molecular weight protein?

I have run 4-12% gel and i got this. I have tried with every gel percentage, still not getting anything,instead like this I got. Can somebody suggest why I am geting such thing...

02 March 2019 6,681 4 View

How to extract highly soluble low molecular wt. protein?

the supernant contains all the low molecular proteins dissolved, tried it with ammonium sulfate ppt, till it reached saturation point, even after that the protein can't be extracted. please suggest.

31 December 2017 2,345 0 View

In ammonium sulfate ppt, in order to check protein's activity, the precipitated pellet is not giving any zone of inhibition. why?

I have checked for activity with 30%, 60%, 80% precipitated pellet suspened in buffer, it didn't show any zone, whereas the treated supernatant such as 30%, 60%, 80% keep on showing zone against...

11 December 2017 9,265 0 View

What is the best fixative agent can be used for low molecular weight protein?

In SDS PAGE.,before staining procedure to be carried out, which one among the solution will be best for protein fixation? (low molecular weight 2kDa to 12kDa) 50% MeOH 50% H2O 25% Acetic acid...

09 October 2017 8,131 3 View

How to store gels for future reference?

After the gel has been properly destained, can it be stored for longer period? How? Kindly Tell me the storage buffer composition. Can we just pause the destaining process and, keep it in some...

09 October 2017 978 0 View

To plot a standard graph, what is the highest concentration of BSA that can be used?

As the unknown O.D i am getting is, 0.559, 1.324, 3.472 etc. what BSA concentration can be used to get a standard graph?

08 September 2017 2,797 1 View

In the first step of protein purificatio,does the protein degrades?the crude protein showed zone of inhibition but the 80%precipitated protein didn't?

why it is not showing zone? for how long the protein remain active after , precipitation, if kept in buffer?

01 January 1970 6,444 2 View

SDS Page - 10, 12, and 15%. Which will give better resolution for low molecular weight protein?

I have run 4-12% gel and i got this. I have tried with every gel percentage, still not getting anything,instead like this I got. Can somebody suggest why I am geting such thing...

01 January 1970 2,000 17 View

How can we choose buffer , for protein extraction/purification?

for low molecular, sensitive, generally shows activity below pH 5. for this kind of peptide(made up of few amino acids), which buffer will be the best? and how to choose? In case of ammonium...

01 January 1970 9,359 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View