If their is no fragmentation of DNA in cancerous cell lines, does it proves their has been no apoptosis?

Where as in case of MTT assay, the results were positive, but in DNA fragmentation assay, their is no fragmentation of genomic DNA can be seen. So, my question is, is the DNA fragmentation solely...

07 August 2019 8,058 7 View

SDS Page - 10, 12, and 15%. Which will give better resolution for low molecular weight protein?

I have run 4-12% gel and i got this. I have tried with every gel percentage, still not getting anything,instead like this I got. Can somebody suggest why I am geting such thing...

02 March 2019 6,681 4 View

Which type of column should i use to check the purity of low molecular weight protein using FPLC reverse phase column chromatography?

03 April 2018 5,925 4 View

How to extract highly soluble low molecular wt. protein?

the supernant contains all the low molecular proteins dissolved, tried it with ammonium sulfate ppt, till it reached saturation point, even after that the protein can't be extracted. please suggest.

31 December 2017 2,345 0 View

In ammonium sulfate ppt, in order to check protein's activity, the precipitated pellet is not giving any zone of inhibition. why?

I have checked for activity with 30%, 60%, 80% precipitated pellet suspened in buffer, it didn't show any zone, whereas the treated supernatant such as 30%, 60%, 80% keep on showing zone against...

11 December 2017 9,265 0 View

What is the best fixative agent can be used for low molecular weight protein?

In SDS PAGE.,before staining procedure to be carried out, which one among the solution will be best for protein fixation? (low molecular weight 2kDa to 12kDa) 50% MeOH 50% H2O 25% Acetic acid...

09 October 2017 8,131 3 View

How to store gels for future reference?

After the gel has been properly destained, can it be stored for longer period? How? Kindly Tell me the storage buffer composition. Can we just pause the destaining process and, keep it in some...

09 October 2017 978 0 View

In the first step of protein purificatio,does the protein degrades?the crude protein showed zone of inhibition but the 80%precipitated protein didn't?

why it is not showing zone? for how long the protein remain active after , precipitation, if kept in buffer?

01 January 1970 6,444 2 View

SDS Page - 10, 12, and 15%. Which will give better resolution for low molecular weight protein?

I have run 4-12% gel and i got this. I have tried with every gel percentage, still not getting anything,instead like this I got. Can somebody suggest why I am geting such thing...

01 January 1970 2,000 17 View

How can we choose buffer , for protein extraction/purification?

for low molecular, sensitive, generally shows activity below pH 5. for this kind of peptide(made up of few amino acids), which buffer will be the best? and how to choose? In case of ammonium...

01 January 1970 9,359 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View