How to extract highly soluble low molecular wt. protein?

Jannatul firdous Siddique @Jannatul_Siddique

01 January 2018 0 2K Report

the supernant contains all the low molecular proteins dissolved, tried it with ammonium sulfate ppt, till it reached saturation point, even after that the protein can't be extracted.

please suggest.

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Jannatul firdous Siddique's questions See All

If their is no fragmentation of DNA in cancerous cell lines, does it proves their has been no apoptosis?

Where as in case of MTT assay, the results were positive, but in DNA fragmentation assay, their is no fragmentation of genomic DNA can be seen. So, my question is, is the DNA fragmentation solely...

07 August 2019 8,179 7 View

SDS Page - 10, 12, and 15%. Which will give better resolution for low molecular weight protein?

I have run 4-12% gel and i got this. I have tried with every gel percentage, still not getting anything,instead like this I got. Can somebody suggest why I am geting such thing...

02 March 2019 6,786 4 View

Which type of column should i use to check the purity of low molecular weight protein using FPLC reverse phase column chromatography?

Hi everyone. I wanna to purify low molecular weight protein with MW of ~3-25 kDa using a FPLC. I know C18 & C8 reverse-phase column (particle size 3 & 5 µm) can be used to separate low...

03 April 2018 6,009 4 View

In ammonium sulfate ppt, in order to check protein's activity, the precipitated pellet is not giving any zone of inhibition. why?

I have checked for activity with 30%, 60%, 80% precipitated pellet suspened in buffer, it didn't show any zone, whereas the treated supernatant such as 30%, 60%, 80% keep on showing zone against...

11 December 2017 9,358 0 View

What is the best fixative agent can be used for low molecular weight protein?

In SDS PAGE.,before staining procedure to be carried out, which one among the solution will be best for protein fixation? (low molecular weight 2kDa to 12kDa) 50% MeOH 50% H2O 25% Acetic acid...

09 October 2017 8,229 3 View

How to store gels for future reference?

After the gel has been properly destained, can it be stored for longer period? How? Kindly Tell me the storage buffer composition. Can we just pause the destaining process and, keep it in some...

09 October 2017 1,096 0 View

To plot a standard graph, what is the highest concentration of BSA that can be used?

As the unknown O.D i am getting is, 0.559, 1.324, 3.472 etc. what BSA concentration can be used to get a standard graph?

08 September 2017 2,906 1 View

In the first step of protein purificatio,does the protein degrades?the crude protein showed zone of inhibition but the 80%precipitated protein didn't?

why it is not showing zone? for how long the protein remain active after , precipitation, if kept in buffer?

01 January 1970 6,556 2 View

SDS Page - 10, 12, and 15%. Which will give better resolution for low molecular weight protein?

I have run 4-12% gel and i got this. I have tried with every gel percentage, still not getting anything,instead like this I got. Can somebody suggest why I am geting such thing...

01 January 1970 2,101 17 View

How can we choose buffer , for protein extraction/purification?

for low molecular, sensitive, generally shows activity below pH 5. for this kind of peptide(made up of few amino acids), which buffer will be the best? and how to choose? In case of ammonium...

01 January 1970 9,459 2 View

Where can I find the recent population of Indian cities (2020s) ?

Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!

10 August 2024 857 4 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View