I tried the snoRNA as my endogenous control, but it's not the best control because the samples showed some differences between the CTs.
Dear Pedro Paulo Gattai
Regarding your question
U6 snRNA will be suitable endogenous control.
(From Exiqon and catlog no:203907)
with regards
pp
You can use RNU6B as endogenous control
Dear Pedro,
I recently answered at length a very similar question. have a look at:
https://www.researchgate.net/post/How_do_I_normalize_the_controls_for_miR_486_in_C2C12_cells
Good luck!
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