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Questions related from Pedro Paulo Gattai
When I studied miRNA expression in normal tissues/cells the control that I used was U6 snRNA. Does someone know if this could be used also with breast cancer cells to estimate miRNA expression in...
04 April 2019 2,047 4 View
If you have a manuscript rejected by a specific journal due to low priority, after respond the reviewer's questions and improve your paper, you will try to submit this to the same journal, or is...
02 February 2018 9,083 9 View
When you are doing research and finds something that seems to be interesting and relevant, do you think, based in the evidences published and your results, that it can be published. But when you...
11 November 2017 6,009 9 View
I have some spin column left over from the kit that originally made to extract total RNA. I want to use these columns to extract microRNA. Is it possible? Has anyone tried it yet?
01 January 2017 9,145 11 View
I tried the Abcam antibody in western blotting, but when I reveal the membrane appear some non specific bands. Even though I left the membrane blocking for 2 hours in 3% albumin solution.
09 September 2016 4,600 9 View
How these eggs does to transpass the blood-brain barrier?
08 August 2016 2,258 0 View
I tried the snoRNA as my endogenous control, but it's not the best control because the samples showed some differences between the CTs.
05 May 2015 9,832 3 View
Besides the method by silica column, is there any other method for extracting microRNA from tissue? What would it be?
09 September 2014 4,279 2 View
I have read that the LNA (locked nucleic acid) is used for in situ hybridization, but this technology also works for qRT-PCR?If so, can I only use the primers to the reaction or use the specific...
09 September 2014 4,823 3 View
As you can see in the image attached, there are four bands. Three are known, one is unknown. Do you know what is it?
08 August 2014 9,497 4 View
As you can see in the image attached, there are four bands. Three are known (genomic DNA band, 28S and 18S rRNA bands), one is unknown. Do you know what it is?
08 August 2014 8,533 10 View
When I'm preparing the samples, after added the SDS-PAGE, the protocol sends to heating these samples at 95°C for 10 minutes. My doubt it is about the integrity of the protein. When I heat the...
01 January 1970 7,092 11 View
I made an extraction of total RNA with mirvana kit of life technologies for read miRNAs from cell samples, occur that the samples stayed with a low concentration (with 200 ng/uL or even less), but...
01 January 1970 6,314 5 View
Why this pattern occurs in gel? I mean, why these bands showed this trajectory? And why appeared that bubble, that I circled in red, in the base of gel?
01 January 1970 1,168 9 View
