Pedro Paulo Gattai

14 Questions 120 Answers 0 Followers

Questions related from Pedro Paulo Gattai

What is the best control miRNA to study miRNA expression in mammalian breast cancer?

When I studied miRNA expression in normal tissues/cells the control that I used was U6 snRNA. Does someone know if this could be used also with breast cancer cells to estimate miRNA expression in...

04 April 2019 2,033 4 View

It is worth submitting a manuscript to the same journal that rejected your paper previously?

If you have a manuscript rejected by a specific journal due to low priority, after respond the reviewer's questions and improve your paper, you will try to submit this to the same journal, or is...

02 February 2018 9,069 9 View

How do not to be cheated when publishing your results?

When you are doing research and finds something that seems to be interesting and relevant, do you think, based in the evidences published and your results, that it can be published. But when you...

11 November 2017 5,995 9 View

Someone knows if I can use the QIAamp RNA spin column of qiagen to extract miRNA, or to extract total RNA with miRNA?

I have some spin column left over from the kit that originally made to extract total RNA. I want to use these columns to extract microRNA. Is it possible? Has anyone tried it yet?

01 January 2017 9,125 11 View

Anyone knows a reliable antibody for rat HGF?

I tried the Abcam antibody in western blotting, but when I reveal the membrane appear some non specific bands. Even though I left the membrane blocking for 2 hours in 3% albumin solution.

09 September 2016 4,582 9 View

Someone knows how the cysticercosis' egg does to reach the brain?

How these eggs does to transpass the blood-brain barrier?

08 August 2016 2,244 0 View

What is the best endogenous control for qRT-PCR of microRNA from rat kidney samples?

I tried the snoRNA as my endogenous control, but it's not the best control because the samples showed some differences between the CTs.

05 May 2015 9,822 3 View

How many methods for extracting microRNA from tissue are there?

Besides the method by silica column, is there any other method for extracting microRNA from tissue? What would it be?

09 September 2014 4,266 2 View

How does it work with the LNA technology for qRT-PCR?

I have read that the LNA (locked nucleic acid) is used for in situ hybridization, but this technology also works for qRT-PCR?If so, can I only use the primers to the reaction or use the specific...

09 September 2014 4,810 3 View

I made a gel of RNA integrity and it showed four bands, what is the mean?

As you can see in the image attached, there are four bands. Three are known, one is unknown. Do you know what is it?

08 August 2014 9,484 4 View

I made a gel for RNA integrity that showed four bands, what does it mean?

As you can see in the image attached, there are four bands. Three are known (genomic DNA band, 28S and 18S rRNA bands), one is unknown. Do you know what it is?

08 August 2014 8,516 10 View

The heating process in preparing samples for running western blotting can separate two subunits of the same protein?

When I'm preparing the samples, after added the SDS-PAGE, the protocol sends to heating these samples at 95°C for 10 minutes. My doubt it is about the integrity of the protein. When I heat the...

01 January 1970 7,081 11 View

What the minimum amount of total RNA that I must use to do cDNA by superscript II RT protocol?

I made an extraction of total RNA with mirvana kit of life technologies for read miRNAs from cell samples, occur that the samples stayed with a low concentration (with 200 ng/uL or even less), but...

01 January 1970 6,304 5 View

Anyone see this on a nitrocellulose membrane?

Why this pattern occurs in gel? I mean, why these bands showed this trajectory? And why appeared that bubble, that I circled in red, in the base of gel?

01 January 1970 1,156 9 View