How many methods for extracting microRNA from tissue are there?

More Pedro Paulo Gattai's questions See All

What is the best control miRNA to study miRNA expression in mammalian breast cancer?

When I studied miRNA expression in normal tissues/cells the control that I used was U6 snRNA. Does someone know if this could be used also with breast cancer cells to estimate miRNA expression in...

03 April 2019 1,953 4 View

It is worth submitting a manuscript to the same journal that rejected your paper previously?

If you have a manuscript rejected by a specific journal due to low priority, after respond the reviewer's questions and improve your paper, you will try to submit this to the same journal, or is...

01 February 2018 8,972 9 View

How do not to be cheated when publishing your results?

When you are doing research and finds something that seems to be interesting and relevant, do you think, based in the evidences published and your results, that it can be published. But when you...

10 November 2017 5,915 9 View

Someone knows if I can use the QIAamp RNA spin column of qiagen to extract miRNA, or to extract total RNA with miRNA?

I have some spin column left over from the kit that originally made to extract total RNA. I want to use these columns to extract microRNA. Is it possible? Has anyone tried it yet?

31 December 2016 9,012 11 View

Anyone knows a reliable antibody for rat HGF?

I tried the Abcam antibody in western blotting, but when I reveal the membrane appear some non specific bands. Even though I left the membrane blocking for 2 hours in 3% albumin solution.

08 September 2016 4,496 9 View

Someone knows how the cysticercosis' egg does to reach the brain?

How these eggs does to transpass the blood-brain barrier?

07 August 2016 2,166 0 View

What is the best endogenous control for qRT-PCR of microRNA from rat kidney samples?

I tried the snoRNA as my endogenous control, but it's not the best control because the samples showed some differences between the CTs.

04 May 2015 9,740 3 View

How does it work with the LNA technology for qRT-PCR?

I have read that the LNA (locked nucleic acid) is used for in situ hybridization, but this technology also works for qRT-PCR?If so, can I only use the primers to the reaction or use the specific...

08 September 2014 4,726 3 View

I made a gel for RNA integrity that showed four bands, what does it mean?

As you can see in the image attached, there are four bands. Three are known (genomic DNA band, 28S and 18S rRNA bands), one is unknown. Do you know what it is?

07 August 2014 8,425 10 View

I made a gel of RNA integrity and it showed four bands, what is the mean?

As you can see in the image attached, there are four bands. Three are known, one is unknown. Do you know what is it?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Ivano Amelio

TRIZOL protocol allows extraction of total RNA content, including small RNAs (microRNAs etc). When there are no specific needs to extract the only small RNAs, TRIZOL is highly recommended, in particular for extraction from tissue specimens.

Fermin Mar-Aguilar

The methods that use a column like miRNeasy kit and miRVana are a good choice. Trizol is good, but we must be careful, because recently was reported that miRNAs with low GC content, are selectively lost during sample preparation rather than degraded in the cell. The small RNA loss occurs when a small number of cells is used for RNA preparation using the standard TRIzol protocol.

Mol Cell. 2012 Jun 29;46(6):893-5. doi: 10.1016/j.molcel.2012.05.036.

Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells.

Kim YK, Yeo J, Kim B, Ha M, Kim VN.