I have read that the LNA (locked nucleic acid) is used for in situ hybridization, but this technology also works for qRT-PCR?
If so, can I only use the primers to the reaction or use the specific plates for qRT-PCR?
Hi Pedro,
If you can elaborate your query it would be more clear. Do you have some specific objective for LNA primers in the qRT-PCR...in my opinion they will turn out to be more expensive for general/routin use
Hi There!
We used LNA to knockdown some RNAs and tested their expression level by qRT-PCR. It works perfectly! You just have to make sure that the primer that you are using for q-PCR do not overlap with the LNA sequence to make sure that the all RNA molecule is degraded. I hope that helps.
Hi Pedro,
Yes, LNA technology works also for qRT-PCR.
Here is a useful link for choosing the probe http://qpcr.probefinder.com/roche3.html
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