How these eggs does to transpass the blood-brain barrier?
When I studied miRNA expression in normal tissues/cells the control that I used was U6 snRNA. Does someone know if this could be used also with breast cancer cells to estimate miRNA expression in...
03 April 2019 2,072 4 View
If you have a manuscript rejected by a specific journal due to low priority, after respond the reviewer's questions and improve your paper, you will try to submit this to the same journal, or is...
01 February 2018 9,110 9 View
When you are doing research and finds something that seems to be interesting and relevant, do you think, based in the evidences published and your results, that it can be published. But when you...
10 November 2017 6,040 9 View
I have some spin column left over from the kit that originally made to extract total RNA. I want to use these columns to extract microRNA. Is it possible? Has anyone tried it yet?
31 December 2016 9,180 11 View
I tried the Abcam antibody in western blotting, but when I reveal the membrane appear some non specific bands. Even though I left the membrane blocking for 2 hours in 3% albumin solution.
08 September 2016 4,632 9 View
I tried the snoRNA as my endogenous control, but it's not the best control because the samples showed some differences between the CTs.
04 May 2015 9,859 3 View
I have read that the LNA (locked nucleic acid) is used for in situ hybridization, but this technology also works for qRT-PCR?If so, can I only use the primers to the reaction or use the specific...
08 September 2014 4,859 3 View
Besides the method by silica column, is there any other method for extracting microRNA from tissue? What would it be?
08 September 2014 4,313 2 View
As you can see in the image attached, there are four bands. Three are known (genomic DNA band, 28S and 18S rRNA bands), one is unknown. Do you know what it is?
07 August 2014 8,572 10 View
As you can see in the image attached, there are four bands. Three are known, one is unknown. Do you know what is it?
07 August 2014 9,531 4 View
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
