Anna Git

6 Questions 139 Answers 0 Followers

Questions related from Anna Git

Can you recommend reliable thin marker pens for lab use?

Sorry, sounds like a low-level question, but I have been searching high and low for years. Marker pens either rub off, wipe off, fade off. Without resorting to labels, is there any brand that...

05 May 2015 7,035 0 View

Is there a DNase that would efficiently cleave DNA in a DNA:RNA hybrid?

I would like to remove the DNA from a DNA:RNA hybrid leaving intact ssRNA (the opposite of RNaseH). DNaseI can do it, but very inefficiently. Is there an alternative to dosing more DNaseI?

04 April 2015 7,273 11 View

How fast do frozen cell lines deteriorate in -80oC compared to liquid nitrogen?

Our protocol for freezing cell lines is to supplement a single-cell suspension with 10% DMSO and slow-freeze overnight at -80oC in a commercial container (e.g. Mr Frosty). The advice is to...

11 November 2014 8,779 6 View

Are there any methods to measure short partly double-stranded RNA concentration that are unaffected by structure?

I have in vitro transcribed a collection of RNAs of 60-100 nt with a substantial proportion of double strandedness (similar to pre-miRNAs). After column-based purification to remove free...

02 February 2014 2,802 1 View

Square-welled tissue culture dishes?

Considering the difficulty in ensuring consistent and homogenous seeding of cells in multi-well dishes and the endless number of threads/advice/voodoo people resort to in order to avoid the cursed...

10 October 2013 2,318 3 View

Double thymidine block of slow growing cell lines?

I am considering cell-cycle-synchronising a slow-dividing cell line (say, 1 week doubling time). Has anyone had any relevant experience? In particular, any suggestions how to relate the...

07 July 2013 5,473 6 View