Greetings everyone,

I am trying to measure the volume of a retinal lesions in an image obtained using OCT. Each section parameters (X and Z parameters) and the distance between each scans are available. I studied a few paper and it seems that Cavalieri principle is the main method that have been used to measure the volume using the area (by ImageJ). I just wanted to be sure that the proposed method is the accurate method so I can measure the volume of lesions.

My scans of retinal lesion are attached.

Proposed method to measure the retinal lesions:

"The volume of a segment, defined as the volume between two contiguous OCT slices, was determined by averaging the area of the lesion in the two slices and multiplying it by the distance (d) separating the slices. Summation of volumetric measurements from each segment allowed calculation of the entire volume of the lesion."

It would be great if you could give me your opinion or any suggestions.

Thank you