How to calculate CFU for Streptomyces as these bacteria grow in the form of mycellial. these are the exospore forming bacteria.
As I am a beginner every time i faced the smearing or uneven bands on gel. What all the factors affecting that affect DNA gel electrophoresis procedure?
26 June 2023 7,781 7 View
I am isolating DNA from Streptomyces species. The problem I am facing is when i am quantifying the DNA by using nanodrop the purity shows 1.8 ratio (260/280) and the concentration was also good...
10 February 2023 1,143 1 View
I want to know that in which quantity if at all needed we should add sodium sulphate to cattle feed, so that we can see increase in milk production and reduction in methane production. Also in how...
21 October 2020 4,803 4 View
Hello friends, i have designed a power amplifier and tested linear parameters in network analyzer and i have extracted S2p file from that. but when i simulate that s2p file in ads again with...
18 January 2020 6,006 0 View
I am designing a 50w power amplifier using ADS tool To simulate that I am using s2p file. I am not getting harmonic balance simulation results properly, can we HB simulation using s2p file or not.
05 November 2019 2,661 3 View
I am designing a power amplifier and want to go for EM simulation for that amplifier.In active components we should add power supply and packaged transistor.but i am not getting how to give power...
06 July 2019 7,820 3 View
Hello, I have designed an LNA in ADS tool and simulated in schematic, and I have got proper results. I want to check momentum results . I had added s2p pad from layout palette added s2p file too,...
02 May 2019 9,812 4 View
As the Financial Inclusion is shaping up globally. I wish to know the further research area to be search for and beneficial for social development.
14 November 2017 2,531 2 View
I am working on application of genetic algorithms in encryption. Can somebody send me a copy or links for good e books related to this topic?
08 October 2013 1,634 5 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View