Waraporn Bunnak Try this online tool: https://www.fruitfly.org/seq_tools/promoter.html

Be sure to select prokaryote as the organism type.

Keep in mind that bacteria often have genes in operons, so the promoter for your gene might be upstream by a number of genes. You can probably deduce if part of an operon if the ORFs are tightly clustered with little space between.

Thank you for adding your answer. Michael J. Benedik David F. Barker