At what temperature the enzymes that split nonstructural carbohydrates in plants are killed?

29 October 2020 6,410 4 View

Finger tapping - which sensor?

We are developing a markerless software for the measurement of bradykinesia (finger tapping) using two monochromatic IR cameras and three infrared LEDs. We would like to validate the results with...

19 October 2020 6,819 2 View

Boiling temperature of 50% ethanol at 6000 m a.s.l.?

18 October 2020 7,718 4 View

What kit use for bacterial dna extraction?

Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...

30 July 2020 6,566 7 View

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)?

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)? Especially, not using Santa-Cruz antibodies? I am being...

04 June 2018 3,394 3 View

Can I detect autophagy via FACS with extracellular staining?

I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...

09 July 2017 1,116 3 View

Hello everybody! Does anyone have experience with isolating bacterial DNA from skin swabs?

23 July 2015 4,748 7 View

Can anybody help with separation of genomic and viral episomal DNA?

I want to get pure genomic fraction and pure episomal fraction without cross-contamination. I want to use them in PCR. I am working with herpesvirus which has quite a large genome. Any suggestions?

09 April 2014 5,111 7 View

Does anyone have experience with separation monosaccharides (neutral and acid) on the Dionex columns?

Which column (CaerboPac MA1, PA1, PA100) is the best? Is it possible to detect neutral and acid monosacchairdes on the one column?

20 September 2013 4,314 1 View

How to increase tissue macrophages

Hello, I am currently in a desperate need of a group brainstorming and I thought I could use your help. I would like to develop an experimental model of human lung cancer and the aim is to test...

01 January 1970 164 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

What is the easiest way to remove the water from organic solvents?

Hello everyone! I have one question. I have couple of solvents and all of them were pure when they were bought but after some months or years of storage after opening I am not 100% sure about...

03 February 2021 2,154 8 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View