Hae Suyash

Betr try wid 20% SDS PAGE gel.Kindly check wid d link provided.

http://www.abcam.com/protocols/electrophoresis-for-western-blot

Hi Patil,

15% gel is well and good for 10kd protein. You can try out 20% gel as well.

However, to get better run on 15% of the gel you can follow some tips!

1. Run the gel at lower voltage (70V) for longer time.

2. Freshly prepare the gel just before you load your samples. This is slightly advantageous over precast gels.

3. Load higher amount (50mg) of protein. So that you can get better band for 10kd protein.

-- Best regards.

For small proteins up to 20 kDa tricine-gels will work better than tris-glycine. In addition, gradient gels (say, 8-18%) will lead to narrower bands. See doi:10.1038/nprot.2006.4, doi:10.1016/0003-2697(87)90587-2