Kishore Ak

3 Questions 29 Answers 0 Followers

Questions related from Kishore Ak

Any standardized protocol for staining the cells which are cultured in 3D culture system?

We are working on staining the specific proteins to check for its localization. We also working of live/dead staining assay simultaneously. Since the cells are embeded in the hydrogel, we could...

02 February 2019 4,476 6 View

What assay could be used to check the cell proliferation in real time?

I'm doing primary cell culture and I want accurate and more sensitive assay to deteremine the cell proliferation in real time. Since I'm using 3D cultute systems, I want an assay which should not...

08 August 2018 3,943 3 View

What could be the reason for thick unusual band on the ladder of p21 western blot.?

There's is a very thick band on the ladder (NOT on the sample lane!) when I tried for p21 western. Is this because of p21 primary antibody (H164).? Is there any measures to get good blots..!

01 January 1970 7,399 3 View