Suyash Bhimgonda Patil

7 Questions 11 Answers 0 Followers

Questions related from Suyash Bhimgonda Patil

What is the best concetration of SDS PAGE gel to visualize 10 kDa protein?

Hello, I am facing problem in visualizing protein of 10 KDa size on 15 % SDS PAGE gel. What are the suggestions for solving this problem? Thanks, Suyash

02 February 2018 3,878 3 View

What if referance genes are also enfluenced by specific treatment in real time PCR? HOw to analyze data in such situation?

I have found that a specific treatment influences the Ct value of both the reference gene (Actin and Ubiquitin) in RT qPCR experiment. Is it possible to analyze such data?

02 February 2018 10,021 4 View

Hello, Anybody working on Pcold GST vector system?

I am unable to induce protein of my interest using pcold GST vector system. I have transformed the vector in BL21 cells. I am unable to see any protein expression on induced culture compared to...

11 November 2017 8,266 6 View

How to calculate annealing temperature for the pcr set up with primers designed for in-fusion cloning?

The primers designed for the infusion cloning system have 15 bp overhang from the vector sequence and around 20 to 25 bp for the sequence from the gene of interest. The question is how to...

05 May 2017 9,122 5 View

What is the reason for variation for transplating shock in different plant species?

Its observed that some plants adapt to transplanting shock easily or even they grow better after being transplanted, while some plants are extremely sensitive for transplanting. What could be the...

02 February 2017 8,363 0 View

Any body have experience using NEB Q5 Site Directed Mutagenesis Kit ?

I am trying to insert 90 bp insertion in 12 KB vector. The insert is a 30 bp tag repeated for 3 times. First I used a 114 bp forward primer and 38 bp reverse primer because there is great chance...

10 October 2016 3,508 11 View

How can I amplify an AT rich genome sequence?

I am trying to amplify an AT rich sequence which is 1543 bp long but still unsuccessful. I have used Phanta Max and Taq enzymes. Please suggest possible solution for it.

07 July 2016 9,063 3 View