What is the best choice of siRNA for rabies virus silence?

Perumal Arumugam Desingu @Perumal-Desingu

08 June 2015 1 414 Report

We are planining to silence Rabies virus by siRNA method what is best gene to silence. please help help us with expert ideas.

Matthias Haberl

Sounds interesting. Depends on which stage you want to interfere. how about the polymerase (L gene)? To just interfere with spreading glycoprotein (G gene).

Badges
Science topic

More Perumal Arumugam Desingu's questions See All

How to increase the transcription efficiency in HEK293 cells using PEI?

Get more than 80% - 90% of transcription efficiency

19 July 2023 7,912 3 View

Is it possible to use In-Circuit Emulation of ARM processor to replicate the Matlab model. Is there any software to put the code into ARM chip?

Is it possible to use In-Circuit Emulation of ARM processor to replicate the Matlab model. Is there any software to put the code into ARM chip? Please suggest on this.

25 April 2023 9,820 2 View

I Want a plagiarism software for my personal use , please share where it available (Link) , price detais?

My own question

15 March 2023 6,905 7 View

What is the difference between feature influence vs feature effect in machine learning (for land use science)?

I’m an ecologist using machine learning algorithms (using the gradient boosting approach for the first time 😊) to gain insight into land use patterns. My question is related to a machine learning...

22 January 2023 5,967 11 View

Is there simple and efficient way or any parametrization method to estimate glacier basal velocity for a valley glacier?

Being an essential parameter, basal velocity estimation of a valley glacier is still not significantly discussed which helps in assessing the glacier water storage capacity over a period and...

02 November 2022 8,153 0 View

Poly Ethylene Glycol 6000 Measurement?

How to measure/convert the Mpa (PEG-6000) to Grams? for example -2.0Mpa = how many grams?

30 October 2022 8,285 3 View

Why the mass conservation ice thickness model approach is superior to the other ice thickness model approaches like GlabTop or velocity-slope?

Most of the studies related to the High mountain glaciers adopt a mass conservation approach at a global level while other approaches are least adopted.

06 February 2022 9,985 0 View

Adhesive for contact lenses ?

Hi Everyone, We need to attach metal beads to contact lenses for a project, and I could not find any adhesive that works with contact lenses (preferably biocompatible) . I know that they are...

01 February 2022 1,887 2 View

In gabbro rock LREE shows enriched pattern pls explain about magma emplacement and tectonic setting of this rock ?

n gabbro rock LREE shows enriched pattern pls explain about magma emplacement and tectonic setting of this roc

13 December 2021 8,321 5 View

Where to get permission to use the Occupational Stress Index in my dissertation?

where to get permission to use the Occupational Stress Index in my dissertation? Can i know about the interpretation?

26 September 2021 6,700 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View