Is there Researchers who are already working on anti-viral surfaces. For ex. anti-viral COVID-19 surfaces ? ?

It would be interesting to know, how the scientific community is contributing to the COVID-19 crisis. How chemical/mechanical/material/metallurgy concepts are being used to produce anti-COVID-19...

16 May 2020 5,141 3 View

How to calculate ECC using HFSS software?

Good morning. Now i am designing MIMO antenna for Ultraband Applications. Can anyone suggest how to plot ECC (Envelope correlation Coefficient) Using HFSS 15 software. Thanks in advance

13 February 2020 5,261 6 View

Data structure and missing values in random forest model?

Hi there, I have over 1 million records, with a categorical response variable and a mix of categorical and continuous predictors. There are several missing values in the dataset, this is...

02 September 2019 494 4 View

Tools/techniques to describe the distribution of cells in a raster, across a study region?

Im trying to analyze and extract patterns from several raster layers, something like what is done for non-raster features. Im interested in learning techniques that describe the distribution of...

02 July 2019 2,333 5 View

Can anyone suggest me how to plot fractional order time delay system in matlab?

I am searching a matlab code for fractional time delay system(continuous case). Still now, i don't got any idea for the above. I need a good suggestion from researcher. Help anyone!!!

06 March 2019 6,097 3 View

Selecting a DC Voltage for EIS Measurements: Can someone help me with this ?

Am confused in deciding the DC Voltage to select for EIS measurement of my ss316L (Coated and Surface modified). Just wanted to crosscheck before proceeding forward. I found this article bit...

12 December 2017 4,913 4 View

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

25 November 2017 8,132 2 View

Why the RNA got degraded during purification ?

29 March 2017 1,127 2 View

Is it possible to amplify a single copy of dna template (practically) efficiently by using PCR ?

14 March 2017 3,999 11 View

Sleep and Vigilance - would you like to submit your next paper to our new journal?

11 December 2016 1,598 0 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View