Perumal Arumugam Desingu

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Questions related from Perumal Arumugam Desingu

What is the ideal way of reconstituting an oligoprobe for fluorescent in situ hybridisation?

We obtained a Fluorescent end labelled oligoprobe for in situ hybridisation from a commercial source to detect viral nucleic acid. To prepare a stock solution from this newly synthesized probe...

11 June 2015 374 3 View

What is best method of next generation sequencing?

I am planning to go for next genetation sequencing in wildlife tissue for detection of viral seguence, so which method of next generation sequencing is better for this.

08 June 2015 3,029 6 View

Is any software available to cross check negative effect of si RNA in host or cell culture?

We designed siRNA for Rabies virus silencing, I need to know the siRNA may cause negative effect of any protein mRNA in mice or not, so before giving this siRNA to mice I need to know. So, please...

08 June 2015 9,323 1 View

Any best software available for metagenomic sequence analysis?

I got sequences by metagenomics method for analysis, I am going by NCBI, BLAST analysis. Any best alternative rapid and good software available to analisis of this sequences.

08 June 2015 8,671 8 View

What is the best choice of siRNA for rabies virus silence?

We are planining to silence Rabies virus by siRNA method what is best gene to silence. please help help us with expert ideas.

08 June 2015 426 1 View

How is loop mediated isothermal amplification (LAMP) working without initial denaturation?

Initial Denaturation step needed to convert double strand DNA to single strand DNA, then primer can bind to the template. First published LAMP paper initial denaturation step was followed, but...

01 June 2015 3,471 9 View