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Questions related from Perumal Arumugam Desingu
We obtained a Fluorescent end labelled oligoprobe for in situ hybridisation from a commercial source to detect viral nucleic acid. To prepare a stock solution from this newly synthesized probe...
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I am planning to go for next genetation sequencing in wildlife tissue for detection of viral seguence, so which method of next generation sequencing is better for this.
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We designed siRNA for Rabies virus silencing, I need to know the siRNA may cause negative effect of any protein mRNA in mice or not, so before giving this siRNA to mice I need to know. So, please...
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I got sequences by metagenomics method for analysis, I am going by NCBI, BLAST analysis. Any best alternative rapid and good software available to analisis of this sequences.
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We are planining to silence Rabies virus by siRNA method what is best gene to silence. please help help us with expert ideas.
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I need to quantify cytockines by real time RT-PCR, what is the best primer for cDNA synthesis, random primer or oligodT or gene specific primer.
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I need to quantify cytokines by real time RT-PCR. I came to known lot of RT enzymes are availalable, among different RT enzymes whice one is good for this work.
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Initial Denaturation step needed to convert double strand DNA to single strand DNA, then primer can bind to the template. First published LAMP paper initial denaturation step was followed, but...
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