Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

What is the best method for preparation of Polymeric nanoparticles for co-delivery?

We are planning to make Polymeric nanoparticles for co-delivery of 2 flavanoides. Which is the best method, that one should follow.

09 February 2021 7,086 8 View

OmniScriptum Publishing Group?

Hello, Has anybody published with OmniScriptum Publishing Group. I got an email from them. The emails claims the editor is from the group but the email is signed out by lambert academic...

08 February 2021 5,611 3 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Following is XRD of iron oxide nano material synthesised using bipyridine derivatives. What are the reasons for Broad Humps?

Even after few repetitions..I am getting similar results. Is is due to organic components?

20 January 2021 9,279 6 View

How much Ti and B (in ppm) is required for effective grain refinement and why?

To have the desired grain size in 6061 and 6063 Al alloy, what should be the minimum % of Ti and B pick up and what is the chemistry/metallurgy behind that?

07 January 2021 9,305 2 View

How to make AlV50% alloy by thermit route?

Kindly provide the technical papers/patents on the production of AlV50% alloy by thermit route, by using V2O5

05 January 2021 2,471 3 View

Apart from deoxidation what are the other applications of Ferro Aluminum (FeAl) and Al wire?

FeAl and Al wire has applications in Steel for deoxidation. I want to know the other application of various grades of FeAl. Does Al wire also has other applications apart from deoxidation? Regards

27 December 2020 8,435 1 View

Is there is any reference if someone is using Ti sponge cored wire instead of FeTi 70% cored wire for plain carbon steel?

There is a different behavior in carbon steel and stainless steel. Drastic low recovery in carbon steel due to high melting temp. But in ss , working temp is high so dissolve at 1668 degree temp....

16 December 2020 8,765 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View