How to minimize non specific bands during PCR?

PCR

06 July 2018 2,507 0 View

Is there any serum-free cell culture media for VERO cell-line growth?

I would like to know if there is any serum free media for the growth of Vero Cell-line

03 April 2018 7,509 3 View

How to set temperatues in hot start or touch down PCR?

Hello All, i would appreciate if any one guide me to set the temperatures to perform the asked PCR method, thanks.

10 November 2017 7,186 5 View

Which is the best primary enrichment broth (Other than BLEB & Fraser Borth) for detection of Listeria monocygenes from food samples?

Hello All, I will appreciate if any one would like to share their experience working on the detection of Listeria monocytogenes from food samples using or used primary enrichment broths other than...

09 October 2017 4,301 3 View

How to minimize formation of non-specific bands (binding) during Polymerase Chain Reaction (PCR)?

Hello All, i would appreciate if someone right there to guide to solve the said problem during PCR reaction. Thanks

09 October 2017 8,146 7 View

Could any one please suggest the best and easy protocol of DNA extraction from E.histolytica & Giardia lamblia?

E.histolytica, Giardia lamblia

08 September 2017 9,254 2 View

Is there any protocol/method to visualize Bacterial samples on SEM without coating?

i am working on Scanning Electron Microscope, i cultured S.aureus on agar, and then took cells and washed them in PBS few times and then fixed over night in 2% Gluteraldehyde and then took a 10...

07 August 2017 7,771 6 View

Can we extract DNA through boiling method from E.histolytica cysts?

I am currently working on DNA extraction from E.histolytica cysts. I used thermofisher DNA extraction kit but it didnt work.

07 August 2017 853 0 View

What is the best route to administer antigenic proteins in mice, for antibodies production?

Hello All, I am working on different pathotypes of E.coli, i separated and characterized different proteins from different pathotypes of E.coli. Now i want to administer those protein to Balb/C...

07 August 2017 2,479 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View