Hello All, I am working on different pathotypes of E.coli, i separated and characterized different proteins from different pathotypes of E.coli. Now i want to administer those protein to Balb/C mice to analyze their antigenicity.
Subcutaneous route is the most commonly used
PCR
06 July 2018 2,507 0 View
I would like to know if there is any serum free media for the growth of Vero Cell-line
03 April 2018 7,509 3 View
Hello All, i would appreciate if any one guide me to set the temperatures to perform the asked PCR method, thanks.
10 November 2017 7,186 5 View
Hello All, currently i am working to culture sheep kidney and testicle cells and i would like to know the best cell culture media for that
09 October 2017 9,879 5 View
Hello All, I will appreciate if any one would like to share their experience working on the detection of Listeria monocytogenes from food samples using or used primary enrichment broths other than...
09 October 2017 4,301 3 View
Hello All, i would appreciate if someone right there to guide to solve the said problem during PCR reaction. Thanks
09 October 2017 8,146 7 View
E.histolytica, Giardia lamblia
08 September 2017 9,254 2 View
i am working on Scanning Electron Microscope, i cultured S.aureus on agar, and then took cells and washed them in PBS few times and then fixed over night in 2% Gluteraldehyde and then took a 10...
07 August 2017 7,771 6 View
I am currently working on DNA extraction from E.histolytica cysts. I used thermofisher DNA extraction kit but it didnt work.
07 August 2017 853 0 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?
25 February 2021 4,075 1 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View