I am currently working on DNA extraction from E.histolytica cysts. I used thermofisher DNA extraction kit but it didnt work.
PCR
06 July 2018 2,612 0 View
I would like to know if there is any serum free media for the growth of Vero Cell-line
03 April 2018 7,615 3 View
Hello All, i would appreciate if any one guide me to set the temperatures to perform the asked PCR method, thanks.
10 November 2017 7,315 5 View
Hello All, currently i am working to culture sheep kidney and testicle cells and i would like to know the best cell culture media for that
09 October 2017 9,991 5 View
Hello All, I will appreciate if any one would like to share their experience working on the detection of Listeria monocytogenes from food samples using or used primary enrichment broths other than...
09 October 2017 4,445 3 View
Hello All, i would appreciate if someone right there to guide to solve the said problem during PCR reaction. Thanks
09 October 2017 8,260 7 View
E.histolytica, Giardia lamblia
08 September 2017 9,360 2 View
i am working on Scanning Electron Microscope, i cultured S.aureus on agar, and then took cells and washed them in PBS few times and then fixed over night in 2% Gluteraldehyde and then took a 10...
07 August 2017 7,873 6 View
Hello All, I am working on different pathotypes of E.coli, i separated and characterized different proteins from different pathotypes of E.coli. Now i want to administer those protein to Balb/C...
07 August 2017 2,582 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View