Could any one please suggest the best and easy protocol of DNA extraction from E.histolytica & Giardia lamblia?

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How to minimize non specific bands during PCR?

PCR

06 July 2018 2,612 0 View

Is there any serum-free cell culture media for VERO cell-line growth?

I would like to know if there is any serum free media for the growth of Vero Cell-line

03 April 2018 7,615 3 View

How to set temperatues in hot start or touch down PCR?

Hello All, i would appreciate if any one guide me to set the temperatures to perform the asked PCR method, thanks.

10 November 2017 7,315 5 View

What is the best Cell Culture media for culturing primary cells?

Hello All, currently i am working to culture sheep kidney and testicle cells and i would like to know the best cell culture media for that

09 October 2017 9,991 5 View

Which is the best primary enrichment broth (Other than BLEB & Fraser Borth) for detection of Listeria monocygenes from food samples?

Hello All, I will appreciate if any one would like to share their experience working on the detection of Listeria monocytogenes from food samples using or used primary enrichment broths other than...

09 October 2017 4,445 3 View

How to minimize formation of non-specific bands (binding) during Polymerase Chain Reaction (PCR)?

Hello All, i would appreciate if someone right there to guide to solve the said problem during PCR reaction. Thanks

09 October 2017 8,260 7 View

Can we extract DNA through boiling method from E.histolytica cysts?

I am currently working on DNA extraction from E.histolytica cysts. I used thermofisher DNA extraction kit but it didnt work.

07 August 2017 966 0 View

Is there any protocol/method to visualize Bacterial samples on SEM without coating?

i am working on Scanning Electron Microscope, i cultured S.aureus on agar, and then took cells and washed them in PBS few times and then fixed over night in 2% Gluteraldehyde and then took a 10...

07 August 2017 7,873 6 View

What is the best route to administer antigenic proteins in mice, for antibodies production?

Hello All, I am working on different pathotypes of E.coli, i separated and characterized different proteins from different pathotypes of E.coli. Now i want to administer those protein to Balb/C...

07 August 2017 2,582 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View