Dr Abdul Samad

10 Questions 13 Answers 0 Followers

Questions related from Dr Abdul Samad

How to minimize non specific bands during PCR?

PCR

07 July 2018 2,580 0 View

Is there any serum-free cell culture media for VERO cell-line growth?

I would like to know if there is any serum free media for the growth of Vero Cell-line

04 April 2018 7,585 3 View

How to set temperatues in hot start or touch down PCR?

Hello All, i would appreciate if any one guide me to set the temperatures to perform the asked PCR method, thanks.

11 November 2017 7,265 5 View

How to minimize formation of non-specific bands (binding) during Polymerase Chain Reaction (PCR)?

Hello All, i would appreciate if someone right there to guide to solve the said problem during PCR reaction. Thanks

10 October 2017 8,221 7 View

What is the best Cell Culture media for culturing primary cells?

Hello All, currently i am working to culture sheep kidney and testicle cells and i would like to know the best cell culture media for that

10 October 2017 9,961 5 View

Which is the best primary enrichment broth (Other than BLEB & Fraser Borth) for detection of Listeria monocygenes from food samples?

Hello All, I will appreciate if any one would like to share their experience working on the detection of Listeria monocytogenes from food samples using or used primary enrichment broths other than...

10 October 2017 4,415 3 View

Could any one please suggest the best and easy protocol of DNA extraction from E.histolytica & Giardia lamblia?

E.histolytica, Giardia lamblia

09 September 2017 9,336 2 View

Can we extract DNA through boiling method from E.histolytica cysts?

I am currently working on DNA extraction from E.histolytica cysts. I used thermofisher DNA extraction kit but it didnt work.

08 August 2017 925 0 View

What is the best route to administer antigenic proteins in mice, for antibodies production?

Hello All, I am working on different pathotypes of E.coli, i separated and characterized different proteins from different pathotypes of E.coli. Now i want to administer those protein to Balb/C...

08 August 2017 2,551 1 View

Is there any protocol/method to visualize Bacterial samples on SEM without coating?

i am working on Scanning Electron Microscope, i cultured S.aureus on agar, and then took cells and washed them in PBS few times and then fixed over night in 2% Gluteraldehyde and then took a 10...

08 August 2017 7,845 6 View