How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

Would anyone have a sample survey questionnaire for an AHP-TOPSIS study?

I am currently working on my undergraduate thesis project and we are trying to compare the AHP method with the AHP-TOPSIS method. I have already found the format for an AHP survey questionnaire,...

03 January 2021 7,213 3 View

Will -80 freezer cause damage to enzymes?

The typical guidance on storing enzymes is to keep them in a -20C freezer, but would storing aliquots of enzyme for long-term storage in a -80C freezer cause damage that would result in the enzyme...

03 December 2020 7,550 3 View

Does anyone experienced EDTA salts precipitation in DMSO?

I added 2 mL of 0.1 M EDTA aqueous solution pH 6, to a solution of 3 mL pure DMSO and instantaneously I observed salt precipitation in solution. Is this happening because the pH of the EDTA...

17 October 2020 6,329 1 View

Did anyone try to measure the thickness of films with fluorescent peptides immobilized by ellipsometry?

I immobilize fluorescent peptide on chitosan films and when I try to measure the thickness of the film with ellipsometry, the software says that I have a film with 2 nm or less. However, with...

01 October 2020 3,702 3 View

Is sytematic review methodology adoped in demographic and population studies?

can a demographer employed the systematic review methodology in their research writing?

19 September 2020 5,242 4 View

How to run Targeted MD simulation using NAMD/ GROMACS?

I want to do targeted MD simulation on a protein (400 amino acids). Can anyone suggest me any good tutorial? Thanks in advance.

17 September 2020 342 2 View

Does anyone sell/know how to make 5 M sodium acetate solution?

I am trying out a new type of binding buffer for DNA extraction and the protocol calls for 5 M sodium acetate, but I cannot find anywhere that sells a stock solution that highly concentrated. The...

13 August 2020 5,149 5 View

Impact of digitalization on Mechanical Engineering?

I am currently researching the impacts of components of digital transformation and Industry 4.0 (optimization, automation, digitalization) on the fairly old field of mechanical engineering. Has...

27 June 2020 2,350 13 View

Impact of data-driven industry and digitalization on Mechanical Engineering field?

I am currently researching the impacts of components of digital transformation and Industry 4.0 (optimization, automation, digitalization) on the fairly old field of mechanical engineering. Has...

24 June 2020 2,360 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View