What is the amount of cells necessary for PIP3 extraction in the case of suspension cells, having as goal to perform a PIP3 Mass ELISA?

20 November 2015 1 5K Report

I am going to use B-ALL cell lines. What is the amount of cells necessary for PIP3 extraction in the case of suspension cells, having as goal to perform a PIP3 Mass ELISA?

Dhivya Sridaran

Dear Mariana,

I think 1 to 3 X 10^6 cells would be sufficient for your extraction. Hope the following link night help you.

https://books.google.co.in/books?isbn=1420069381

Badges
Science topic

More Mariana Oliveira's questions See All

Can holes for pressure probes in journal bearings modify the original pressure distribution?

It is very common to measure the pressure distribution inside the bearings with pressure probes connected to the oil film through holes in the bush. Once filled and pressurized, the probe lines...

12 June 2024 1,936 0 View

Why do you translate my name?

My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.

27 May 2024 8,228 2 View

Anyone who knows how to program in Fortran or similar language who can access the complementary Incomplete Gamma function?

Hello Does anyone know how to program in Fortran or any other language that uses advanced calculation libraries? I need help making an executable to obtain results from 2 equations that use the...

20 May 2024 6,610 5 View

Is it possible that the binding energy of heavy quarkonia becomes more and more negative as the temperature increases?

I saw in many articles that quarkonia has negative binding energy and at some places it also has positive value but the pattern of binding energy is always decreasing...... Can anyone explain this...

15 May 2024 6,918 0 View

Where can I download the newest version of AvSoft Bioview image analysis software?

AvSoft Bioview is an image analysis software that allows you to mark parts of an image based on the color of the pixels. Once you've marked them, it gives a number that shows exactly how many...

09 May 2024 7,239 0 View

How can I set the distance between peptide and bilayer using Packmol?

I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...

29 April 2024 8,231 1 View

Why we are solving the Schrodinger equation for quarkonium system in N-dimensional space, while the quarkonium is a three-dimensional system?

If we use the medium modified Cornell type potential which has both the Coulombic and linear terms which is three dimensional. What is the main advantage of solving the Schrödinger equation in N...

27 April 2024 3,920 0 View

How to calculate the solute concentration in molecular dynamics simulation?

I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...

16 April 2024 2,051 2 View

Problems with conventional pcr. What can it be?

I have a problem with conventional PCR electrophoresis, we are analyzing the deletion of ccr5 delta 32. And this material started to cause problems recently, there was no change in the protocol...

03 April 2024 3,896 3 View

How to obtain the standard deviation of multiple sample scans in FTIR ATR Cary 630?

Hello! Does anyone know where can I find the standard deviation out of the multiple scans done in FTIR ATR using Cary 630? For my method, I've selected 64 sample scans, so as to improve the signal...

02 April 2024 9,690 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View