Mariana Oliveira

3 Questions 1 Answers 0 Followers

Questions related from Mariana Oliveira

What is the best method/technique to assess Smac release from mitochondria in leukemic cells?

I found a poster presentation of a FACS-based Smac/DIABLO release assay, which would be a good approach for leukemic cells, but I can't find the protocol. Does anyone have it? Another approach...

30 May 2023 6,460 0 View

What is the amount of cells necessary for PIP3 extraction in the case of suspension cells, having as goal to perform a PIP3 Mass ELISA?

I am going to use B-ALL cell lines. What is the amount of cells necessary for PIP3 extraction in the case of suspension cells, having as goal to perform a PIP3 Mass ELISA?

20 November 2015 4,619 1 View

Does anyone have experience in detecting PIP3 levels by flow cytometry?

I would like to have a protocol to follow.

20 November 2015 3,979 1 View