What is best approch available for small RNA (miRNA and pi RNA) quantification without any kit ?

best possible approch for small RNA quantification

31 December 2017 3,421 4 View

this is query regarding small RNA RNAseq (miRNA and piRNA) using NEB kit. i am putting my bioanalyser electropherogram ?

need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...

10 November 2017 593 3 View

How to design your Chip experiment based upon your DNA modifications data ?

how to design your Chip experiment based upon your DNA modifications data ?

31 December 2016 2,947 0 View

What is best and reliable sofware, database or website to find transcription ?

What is best and reliable software, database or website to find transcription factor binding site ?for given genomic streach? Editwhat is best and reliable sofware, database or website to find...

11 December 2016 7,955 0 View

What is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic streach?

what is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic stretch?

11 December 2016 9,314 0 View

How to design primers for Chromatin conformation capture (3C)?

basically how to plan for this experiment because how one can targate a genomic location as enhancer for a gene of interest?

07 August 2016 7,298 11 View

Is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

04 May 2016 8,626 6 View

Can i have real time primer sets for Hdac2,5,7 in case of mouse?

I am looking for these primer sets or their sequence from any published article ?

04 May 2016 8,176 0 View

Do we have any specific inhibitor known for TET 1,2 ,3 ?

04 May 2016 3,759 1 View

-ve RT is important in case of small RNA amplification using total RNA. How to get rid of similar amplification in this case

Small RNA

01 January 1970 3,825 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View