I am looking for these primer sets or their sequence from any published article ?
best possible approch for small RNA quantification
31 December 2017 3,529 4 View
need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...
10 November 2017 705 3 View
how to design your Chip experiment based upon your DNA modifications data ?
31 December 2016 3,029 0 View
is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?
31 December 2016 1,601 2 View
what is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic stretch?
11 December 2016 9,398 0 View
What is best and reliable software, database or website to find transcription factor binding site ?for given genomic streach? Editwhat is best and reliable sofware, database or website to find...
11 December 2016 8,041 0 View
basically how to plan for this experiment because how one can targate a genomic location as enhancer for a gene of interest?
07 August 2016 7,425 11 View
is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?
04 May 2016 8,716 6 View
i am looking for inhibitors of these enzyme known to demethylate DNA?
04 May 2016 3,841 1 View
Small RNA
01 January 1970 3,927 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View