Small RNA
best possible approch for small RNA quantification
31 December 2017 3,529 4 View
need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...
10 November 2017 705 3 View
how to design your Chip experiment based upon your DNA modifications data ?
31 December 2016 3,029 0 View
is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?
31 December 2016 1,601 2 View
what is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic stretch?
11 December 2016 9,398 0 View
What is best and reliable software, database or website to find transcription factor binding site ?for given genomic streach? Editwhat is best and reliable sofware, database or website to find...
11 December 2016 8,041 0 View
basically how to plan for this experiment because how one can targate a genomic location as enhancer for a gene of interest?
07 August 2016 7,425 11 View
is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?
04 May 2016 8,716 6 View
I am looking for these primer sets or their sequence from any published article ?
04 May 2016 8,263 0 View
i am looking for inhibitors of these enzyme known to demethylate DNA?
04 May 2016 3,841 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
