What is best approch available for small RNA (miRNA and pi RNA) quantification without any kit ?

best possible approch for small RNA quantification

31 December 2017 3,421 4 View

this is query regarding small RNA RNAseq (miRNA and piRNA) using NEB kit. i am putting my bioanalyser electropherogram ?

need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...

10 November 2017 593 3 View

How to design your Chip experiment based upon your DNA modifications data ?

how to design your Chip experiment based upon your DNA modifications data ?

31 December 2016 2,947 0 View

Is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?

is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?

31 December 2016 1,522 2 View

What is best and reliable sofware, database or website to find transcription ?

What is best and reliable software, database or website to find transcription factor binding site ?for given genomic streach? Editwhat is best and reliable sofware, database or website to find...

11 December 2016 7,955 0 View

What is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic streach?

what is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic stretch?

11 December 2016 9,314 0 View

How to design primers for Chromatin conformation capture (3C)?

basically how to plan for this experiment because how one can targate a genomic location as enhancer for a gene of interest?

07 August 2016 7,298 11 View

Is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

04 May 2016 8,626 6 View

Can i have real time primer sets for Hdac2,5,7 in case of mouse?

I am looking for these primer sets or their sequence from any published article ?

04 May 2016 8,176 0 View

Do we have any specific inhibitor known for TET 1,2 ,3 ?

04 May 2016 3,759 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View