What is best approch available for small RNA (miRNA and pi RNA) quantification without any kit ?

best possible approch for small RNA quantification

31 December 2017 3,421 4 View

this is query regarding small RNA RNAseq (miRNA and piRNA) using NEB kit. i am putting my bioanalyser electropherogram ?

need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...

10 November 2017 593 3 View

Is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?

is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?

31 December 2016 1,522 2 View

What is best and reliable sofware, database or website to find transcription ?

What is best and reliable software, database or website to find transcription factor binding site ?for given genomic streach? Editwhat is best and reliable sofware, database or website to find...

11 December 2016 7,955 0 View

What is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic streach?

what is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic stretch?

11 December 2016 9,314 0 View

How to design primers for Chromatin conformation capture (3C)?

basically how to plan for this experiment because how one can targate a genomic location as enhancer for a gene of interest?

07 August 2016 7,298 11 View

Is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

04 May 2016 8,626 6 View

Can i have real time primer sets for Hdac2,5,7 in case of mouse?

I am looking for these primer sets or their sequence from any published article ?

04 May 2016 8,176 0 View

Do we have any specific inhibitor known for TET 1,2 ,3 ?

04 May 2016 3,759 1 View

-ve RT is important in case of small RNA amplification using total RNA. How to get rid of similar amplification in this case

Small RNA

01 January 1970 3,825 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View