What is best approch available for small RNA (miRNA and pi RNA) quantification without any kit ?

best possible approch for small RNA quantification

31 December 2017 3,421 4 View

this is query regarding small RNA RNAseq (miRNA and piRNA) using NEB kit. i am putting my bioanalyser electropherogram ?

need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...

10 November 2017 593 3 View

How to design your Chip experiment based upon your DNA modifications data ?

how to design your Chip experiment based upon your DNA modifications data ?

31 December 2016 2,947 0 View

Is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?

is it possible to colocalize two RNA mol using Fluorocent like methods as we colocalize two proteins in case of immunostaining ?

31 December 2016 1,522 2 View

What is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic streach?

what is best and reliable sofware, database or website to find transcription factor binding site ?for given genomic stretch?

11 December 2016 9,314 0 View

How to design primers for Chromatin conformation capture (3C)?

basically how to plan for this experiment because how one can targate a genomic location as enhancer for a gene of interest?

07 August 2016 7,298 11 View

Is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

is it possible to do real time PCR for detection of pre/pri miRNA in given tissue type using syber green ?

04 May 2016 8,626 6 View

Can i have real time primer sets for Hdac2,5,7 in case of mouse?

I am looking for these primer sets or their sequence from any published article ?

04 May 2016 8,176 0 View

Do we have any specific inhibitor known for TET 1,2 ,3 ?

04 May 2016 3,759 1 View

-ve RT is important in case of small RNA amplification using total RNA. How to get rid of similar amplification in this case

Small RNA

01 January 1970 3,825 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View