Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View

Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Thawing or defrosting methods for frozen soil samples?

I have soil samples that have been frozen for a while and I need to re-acclimate them for a series of analyses that require incubation at 25C. I have been looking for laboratory methods but seem...

04 January 2021 4,862 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View