Laura Walker Simpson

3 Questions 2 Answers 0 Followers

Questions related from Laura Walker Simpson

How can I prevent evaporation in a 48 hr microplate fluorescent spectrophotometer experiment held at 37C?

Since my samples are only 200 ul they evaporate quickly if left at 37C in the plate reader. I tried PerkinElmer TopSeal-A Plus membranes to seal the wells and hold in the moisture. They say they...

25 March 2016 656 2 View

Can SDS be used to retain beta amyloid monomers and will this impact their mobility?

I need a control of beta amyloid monomers but I am using FCS and will be determining the size through the diffusion coefficient of the fluorescently labeled proteins. Therefore the monomers need...

04 March 2016 5,441 1 View

What is a suitable substrate to culture PC12 cells?

I have been using the PC12 cells that require a substrate for adherence. I have used collagen I coated on glass cover slips but have found the cells to not adhere very well. I have read that...

01 June 2015 8,151 3 View