Laura Walker Simpson

7 Questions 6 Answers 0 Followers

Questions related from Laura Walker Simpson

What protocol should be followed to label free protein on a TEM grid with immunogold?

I am trying to look at the binding location of conformation specific antibodies for the beta amyloid protein (antibodies: A11, LOC, & 6E10). I am aggregating the protein in solution then...

18 September 2018 7,147 7 View

I am having problems monomerizing Beta Amyloid 42, I am following published protocols but my dissolved solution is cloudy, can someone help me please?

I use to monomerize my Abeta 42 (Anaspec) in HFIP, dry to a peptide film and store it at -20C. To prepare for an experiment I would dissolve in NaOH. I would see a lag period doing ThT assays...

21 October 2016 1,021 3 View

How can I prevent evaporation in a 48 hr microplate fluorescent spectrophotometer experiment held at 37C?

Since my samples are only 200 ul they evaporate quickly if left at 37C in the plate reader. I tried PerkinElmer TopSeal-A Plus membranes to seal the wells and hold in the moisture. They say they...

25 March 2016 678 2 View

Can SDS be used to retain beta amyloid monomers and will this impact their mobility?

I need a control of beta amyloid monomers but I am using FCS and will be determining the size through the diffusion coefficient of the fluorescently labeled proteins. Therefore the monomers need...

04 March 2016 5,473 1 View

Will Thioflavin T assay bind to agarose gels?

I want to look at protein aggregation in a hydrogel but Thioflavin T binds to protein aggregates. This means I can't use collagen. I want to find out if agarose, hylaurionic acid or other...

02 July 2015 1,089 0 View

Do SH-SY5Y cells remain differentiated once exposed to IGF or RA?

I would like to differentiate a population of SH-SY5Y cells and then lift them from the culture substrate and mix them into a collagen gel with beta amyloid. I would let them differentiate in the...

15 June 2015 5,736 2 View

What is a suitable substrate to culture PC12 cells?

I have been using the PC12 cells that require a substrate for adherence. I have used collagen I coated on glass cover slips but have found the cells to not adhere very well. I have read that...

01 June 2015 8,189 3 View