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Questions related from Laura Walker Simpson
I am trying to look at the binding location of conformation specific antibodies for the beta amyloid protein (antibodies: A11, LOC, & 6E10). I am aggregating the protein in solution then...
18 September 2018 7,147 7 View
I use to monomerize my Abeta 42 (Anaspec) in HFIP, dry to a peptide film and store it at -20C. To prepare for an experiment I would dissolve in NaOH. I would see a lag period doing ThT assays...
21 October 2016 1,021 3 View
Since my samples are only 200 ul they evaporate quickly if left at 37C in the plate reader. I tried PerkinElmer TopSeal-A Plus membranes to seal the wells and hold in the moisture. They say they...
25 March 2016 678 2 View
I need a control of beta amyloid monomers but I am using FCS and will be determining the size through the diffusion coefficient of the fluorescently labeled proteins. Therefore the monomers need...
04 March 2016 5,473 1 View
I want to look at protein aggregation in a hydrogel but Thioflavin T binds to protein aggregates. This means I can't use collagen. I want to find out if agarose, hylaurionic acid or other...
02 July 2015 1,089 0 View
I would like to differentiate a population of SH-SY5Y cells and then lift them from the culture substrate and mix them into a collagen gel with beta amyloid. I would let them differentiate in the...
15 June 2015 5,736 2 View
I have been using the PC12 cells that require a substrate for adherence. I have used collagen I coated on glass cover slips but have found the cells to not adhere very well. I have read that...
01 June 2015 8,189 3 View