21 Questions 10 Answers 0 Followers
Questions related from Gloria Bora Kim
Hi all, I'm trying to get a cell distruption vessel such as this one (Parr Instrument 4635,...
19 November 2019 4,598 1 View
I'm trying to quantify the amount of DNA in adherent cells, does anyone have a good protocol for cell lysis to prepare samples for Picogreen DNA assay? Any help will be greatly appreciated! Gloria
13 May 2016 10,049 2 View
Does anyone have # of adherent cells to seed in 48 well for 7-day DNA quantification assay to study cell proliferation? I would imaging the seeding # will be slightly different from...
13 May 2016 5,062 1 View
Both beta-actin and beta-tubulin are frequently used as load controls in WB, but can I measure the amount of beta tubulin in my sample and normalized with respect to beta-actin? In other words,...
12 May 2016 650 2 View
Would schwannoma cell line show different levels of S100-beta proteins depending on their stage of differentation? or are they terminally differentiated already so no matter how you treat them...
29 April 2016 5,131 1 View
as a brain cancer drug, which one is more commonly used in drug delivery systems?
23 March 2016 9,169 2 View
I am wondering if F-12K media would affect cell growth of Schwann cells, as compared to DMEM normally used for Schwann cells I have. Thanks! Gloria
12 August 2015 2,321 1 View
I heard most brain cancer cells avoid t-cells very well, but is there a cell line (human or animal) that is a little more vulnerable to T-cells than others? thanks! Gloria
09 August 2015 6,247 3 View
Does anyone know why these PC12 cells of one picture look a little rough on their surfaces compared to the other one? They look slightly different from usual and I can't figure out why..Humidity...
27 July 2015 5,854 5 View
A TEER electrode is typically used to test the integrity of endothelial cell layers. Does anyone know how to design your own TEER electrode using things like a volt meter? Thanks, Gloria
29 June 2015 7,669 4 View
Other than using a portable incubator, is there a way to transfer cells without freezing for many hours?
21 May 2014 1,821 13 View
What is a solvent for folic acid?
07 May 2014 6,259 16 View
I would like to know if you can get more than 100% cell viability using MTT assays. Thanks!
31 March 2014 7,968 5 View
Any material good for enhancing platinum adhering on hydrophobic surfaces?
18 June 2013 2,360 2 View
Does anyone know how to measure sheet resistance of a material (polymer solution) using potentiostat?
20 March 2013 9,636 12 View
I would like to measure concentration of BCNU anticancer drug in PBS solution using UV. For some reason, my peak is really broad (peak almost near 340nm) with band width over 200nm and it has a...
29 October 2012 8,225 4 View
Can I dissolve water-soluble PEDOT:PSS nanoparticle solution in chloroform?
26 September 2012 1,080 6 View
PEO is water soluble, but how do you dissolve PEG in organic solvent such as chloroform?
24 September 2012 6,233 21 View
which neuronal cell line is RELATIVELY the cheapest (in terms of cell price itself+medium+additives) and easiest to handle and culture? But still representative to study the interaction between...
21 July 2012 6,769 2 View
Amperometric biosensors display changes in current corresponding to changes in the concentration of an analyte. For in vitro biosensing where analytes will be provided to a batch of PBS for...
27 June 2012 9,753 9 View
fast scan cyclic voltammetry seems to be the best available tool for sensing neurochemicals (such as glucose, dopamine) while providing excellent sensitivity and specificity, then why do...
15 May 2012 4,970 3 View
