Can a sample positive for E. canis by smear test, be negative by PCR test?

We received three Dog blood samples for E. canis PCR test. The DNA was extracted using QIAamp DNA blood mini kit and sujected to PCR. The template DNA was subjected to two different PCR using...

11 December 2019 6,801 7 View

Any suggestion for low cost Real-Time PCR?

I am planning to buy a real time PCR instrument for my laboratory in Chennai, India. Budget is my primary concern and I am in a great dilemma to decide which machine might be the best choice for...

07 August 2019 8,015 3 View

Discrepency between nanodrop reading and agarose gel electrophoresis interpretation?

06 July 2019 1,294 10 View

What can be the possible reason for the signal observed in NTC during TaqMan probe based qPCR (Real Time PCR) assay?

Hello everyone, I am carrying out TaqMan probe based Real-Time PCR assay. I am able to observe signal peak for the NTC sample. However upon agarose electrophoresis I was unable to see any band...

06 July 2019 1,392 7 View

Which TaqMan dye combination is best suited for SNP genotyping?

I am planning to standardize a TaqMan duplex PCR assay for allelic discrimination. I intend to use FAM and TAMRA for one probe. Which dye and quencher would be best for the second probe that can...

03 April 2019 5,418 3 View

Why fungal DNA does not yield amplification against ITS-1 and ITS-4 primers?

I tried extracting DNA from an unknown environmental fungal isolate using Qiagen DNeasy extraction kit. However, I was unable to extract DNA in the first attempt. Then I tried increasing the...

02 March 2019 4,917 13 View

What could be the reason for non specific amplification of gDNA upon serial dilution?

The aim of my study was to evaluate the sensitivity the PCR assay for the in-house primers that I had designed. The concentration of the extracted DNA was 564.4 ng/uL with A260/A280 ratio of 1.97...

01 February 2019 185 2 View

Is it possible to differentiate plant tissues based on bisulfite sequencing methodology?

My objective is to identify the tissue of origin used for the plant extracts preparation. Is it possible to extract DNA from any provided plant extracts and subject it to bisulfite sequencing and...

01 February 2019 210 3 View

How upload a sequence with novel mutation in GenBank?

04 May 2016 4,605 6 View

Muller-Hinton agar contains ferrous or ferric ion?

I want to know the oxidation state of the iron ion present in the Muller-Hinton agar. Whether it is ferrous ion or ferric ion?

04 May 2016 1,886 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View