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Questions related from Yugendran T
We received three Dog blood samples for E. canis PCR test. The DNA was extracted using QIAamp DNA blood mini kit and sujected to PCR. The template DNA was subjected to two different PCR using...
12 December 2019 6,904 7 View
I am planning to buy a real time PCR instrument for my laboratory in Chennai, India. Budget is my primary concern and I am in a great dilemma to decide which machine might be the best choice for...
08 August 2019 8,119 3 View
Hello everyone, I am carrying out TaqMan probe based Real-Time PCR assay. I am able to observe signal peak for the NTC sample. However upon agarose electrophoresis I was unable to see any band...
07 July 2019 1,530 7 View
I tried extracting DNA from Garcinia fruit and got the following readings with Nanodrop. Nucleic acid: 149.7 ng/µL A260/A280: 1.39 A260/A230: 0.12 A260: 2.99 A280: 2.15 Factor: 50 Baseline...
07 July 2019 1,404 10 View
I am planning to standardize a TaqMan duplex PCR assay for allelic discrimination. I intend to use FAM and TAMRA for one probe. Which dye and quencher would be best for the second probe that can...
04 April 2019 5,523 3 View
I tried extracting DNA from an unknown environmental fungal isolate using Qiagen DNeasy extraction kit. However, I was unable to extract DNA in the first attempt. Then I tried increasing the...
03 March 2019 5,004 13 View
My objective is to identify the tissue of origin used for the plant extracts preparation. Is it possible to extract DNA from any provided plant extracts and subject it to bisulfite sequencing and...
02 February 2019 293 3 View
The aim of my study was to evaluate the sensitivity the PCR assay for the in-house primers that I had designed. The concentration of the extracted DNA was 564.4 ng/uL with A260/A280 ratio of 1.97...
02 February 2019 277 2 View
I want to know the oxidation state of the iron ion present in the Muller-Hinton agar. Whether it is ferrous ion or ferric ion?
05 May 2016 1,981 2 View
I have sequence with a novel mutation at a particular nucleotide position within the gene encoding sequence. How can I submit this particular sequence to GenBank and obtain the accession number?
05 May 2016 4,707 6 View
The Melt-Curve analysis are based on the GC content of the sequence. But, in case of 'T' to 'A' mutation or 'A' to 'T' mutation there is no change in GC content of the sequence. Then how can we...
07 July 2015 332 8 View
I am planning to standardise a qpCR assay for gram negative bacteria. DNA extracted by boiling lysis method works fine for conventional PCR but, will it hamper qPCR assay as it is very sensitive?...
06 June 2015 5,353 7 View
I intend to carry out relative gene expression analysis for genes conferring antibiotic resistance using Taqman based qPCR assay. I want to quantify the mRNA level from E. coli and K. pneumoniae...
04 April 2015 9,089 3 View
How to separate an enzyme from its cofactor?
03 March 2015 7,259 5 View
I need to receive bacterial control strains from a lab in the USA. So what are the MTA guidelines for doing this?
11 November 2014 4,697 1 View
I am working on a transmembrane protein. It is an efflux pump which belongs to MFS super family. I want to predict its structure and conduct docking studies. What are different methods for...
08 August 2014 2,143 9 View
I am working with DNA binding proteins. I need to show that the protein binds with the DNA experimentally. What is a simple method for doing the same with minimum resources available in the...
01 January 2014 7,839 2 View
I have predicted a protein structure based on peptide sequence, using SwissModel, Phyre2 and M4T Server ver. 3.0. All the three structures predicted by these software have minute variation. I...
08 August 2013 9,584 10 View
I have three different amino acid sequences for a particular protein of interest. The peptide sequences are differing in number of aa residues at N terminal end. Will the extra aa residue on the...
08 August 2013 3,264 22 View
