Muller-Hinton agar contains ferrous or ferric ion?

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Can a sample positive for E. canis by smear test, be negative by PCR test?

We received three Dog blood samples for E. canis PCR test. The DNA was extracted using QIAamp DNA blood mini kit and sujected to PCR. The template DNA was subjected to two different PCR using...

11 December 2019 6,949 7 View

Any suggestion for low cost Real-Time PCR?

I am planning to buy a real time PCR instrument for my laboratory in Chennai, India. Budget is my primary concern and I am in a great dilemma to decide which machine might be the best choice for...

07 August 2019 8,159 3 View

Discrepency between nanodrop reading and agarose gel electrophoresis interpretation?

I tried extracting DNA from Garcinia fruit and got the following readings with Nanodrop. Nucleic acid: 149.7 ng/µL A260/A280: 1.39 A260/A230: 0.12 A260: 2.99 A280: 2.15 Factor: 50 Baseline...

06 July 2019 1,438 10 View

What can be the possible reason for the signal observed in NTC during TaqMan probe based qPCR (Real Time PCR) assay?

Hello everyone, I am carrying out TaqMan probe based Real-Time PCR assay. I am able to observe signal peak for the NTC sample. However upon agarose electrophoresis I was unable to see any band...

06 July 2019 1,577 7 View

Which TaqMan dye combination is best suited for SNP genotyping?

I am planning to standardize a TaqMan duplex PCR assay for allelic discrimination. I intend to use FAM and TAMRA for one probe. Which dye and quencher would be best for the second probe that can...

03 April 2019 5,565 3 View

Why fungal DNA does not yield amplification against ITS-1 and ITS-4 primers?

I tried extracting DNA from an unknown environmental fungal isolate using Qiagen DNeasy extraction kit. However, I was unable to extract DNA in the first attempt. Then I tried increasing the...

02 March 2019 5,038 13 View

What could be the reason for non specific amplification of gDNA upon serial dilution?

The aim of my study was to evaluate the sensitivity the PCR assay for the in-house primers that I had designed. The concentration of the extracted DNA was 564.4 ng/uL with A260/A280 ratio of 1.97...

01 February 2019 324 2 View

Is it possible to differentiate plant tissues based on bisulfite sequencing methodology?

My objective is to identify the tissue of origin used for the plant extracts preparation. Is it possible to extract DNA from any provided plant extracts and subject it to bisulfite sequencing and...

01 February 2019 329 3 View

How upload a sequence with novel mutation in GenBank?

I have sequence with a novel mutation at a particular nucleotide position within the gene encoding sequence. How can I submit this particular sequence to GenBank and obtain the accession number?

04 May 2016 4,747 6 View

How can we identify T to A mutation using High Resolution Melting analysis?

The Melt-Curve analysis are based on the GC content of the sequence. But, in case of 'T' to 'A' mutation or 'A' to 'T' mutation there is no change in GC content of the sequence. Then how can we...

06 July 2015 377 8 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View