Stefan Gajewski

2 Questions 141 Answers 0 Followers

Questions related from Stefan Gajewski

How can I obtain the extinction coefficient for hair pinned DNA oligos?

I am trying to get very accurate concentration measurements for DNA substrates. For ssDNA I get reliable sequence based extinction coefficients from the manufacturer. Introducing a hair pin of a...

06 June 2014 5,497 6 View

Is it possible to separate a 500 kDa protein complex from a 525kDa protein complex ?

I am about to purify a protein complex and have a difficult problem. Let's say protein A, B and C with 100kDa, 80kDa and 25kDa are the components. I know that they form inhomogeneous complexes...

04 April 2013 3,839 23 View