Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Pseudo random number generator in Matlab code ?

i want to implement pseudo random number generator in matlab ...i request to every one if someone have code these PRNG share with me SplitMix Permuted Congruential Generator (PCG) Random...

14 February 2021 6,828 7 View

Please ,How can I correct this kind of error?

27 January 2021 4,815 5 View

What are the standard parameter values of the commonly used classifiers?

What are the standard parameter values of the commonly used classifiers such as Support-vector machine, k-nearest neighbors, Decision tree, Random forest?

06 January 2021 6,940 7 View

PDF of a Book?

Can anyone provide me the PDF for Distributed Energy Resources in Microgrids (Integration, Challenges and Optimization)?It would be really helpful

27 December 2020 8,015 3 View

Simulation of Smart Grid?

Somepne please help me where to learn the simulation of Smart Grid on YouTube or other bookr esources?

22 December 2020 421 3 View

Doping of r-GO?

if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...

21 December 2020 6,091 3 View

XRD peaks for gold nanoparticles ?

is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...

17 December 2020 9,275 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View