How do you determine concentration of bound protein using Bradford Assay Method?

Hello, I am currently using LDH and bounding to toyo pearl chromatographic particles. I know I can determine the concentration of the protein in a buffer solution using the Bradford Assay Method....

11 December 2015 8,553 7 View

What is the diameter of LDH?

10 November 2015 952 0 View

How to determine protein concentration in a solution?

10 November 2015 3,456 0 View

How do we measure the concentration of immoblized LDH protein?

hello, I am currently using LDH protein and have so far made a mixture of the LDH solution (in a phosphate buffer) and toyopearl chromatograph particles. Now, I want to determine how much of the...

10 November 2015 1,475 3 View

How to determine how particles are inside in a PEEK column?

09 October 2015 5,539 2 View

What is the Size of LDH protein?

09 October 2015 2,202 1 View

How do to calculate dead volume?

Hello, I was wondering if any one knew how I can determine the dead volume in HPLC run. Is there any particular output I am looking for from the HPLC?

09 October 2015 6,408 3 View

How do I prepare a 0.1M acetate buffer?

08 September 2015 855 8 View

Why treat proteins with different buffers?

Hello, i am currently doing a research which involves the use of proteins,buffers and HPLC. I am planning on using acetate buffer for the entirety of the research. However, the researches i am...

08 September 2015 394 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View