Is a acetate buffer a good buffer system to use for LDH? i know acetate buffer has a range of 3-6. is this a good enough buffer. if not what is a good buffer system for LDH?
Hello, I am currently using LDH and bounding to toyo pearl chromatographic particles. I know I can determine the concentration of the protein in a buffer solution using the Bradford Assay Method....
11 December 2015 8,698 7 View
Hello Everyone, I was wondering if the diameter of LDH is determined in a research paper? if someone can point me to that research paper or web page, i would really appreciate it. Thank you.
10 November 2015 1,008 0 View
Hello, If i did reaction in order to attached a protein to a chromatographic particles and if i prepare a standard curve and determine the concentration and i further react the protein and...
10 November 2015 3,508 0 View
hello, I am currently using LDH protein and have so far made a mixture of the LDH solution (in a phosphate buffer) and toyopearl chromatograph particles. Now, I want to determine how much of the...
10 November 2015 1,528 3 View
Hello all, When packing a PEEK column, one that's is opaque. Is there a technique or method used to determine how much particles are packed inside? Is there a way to determine that? thank you
09 October 2015 5,595 2 View
Hello, I was wondering if any one knew how I can determine the dead volume in HPLC run. Is there any particular output I am looking for from the HPLC?
09 October 2015 6,461 3 View
I was wondering what the size of the LDH protein is?
09 October 2015 2,331 1 View
Hello, I am in the process of making mobile phases for my HPLC research and I had one question about buffer. The way I prepared my 0.1M acetate buffer was as follows. For the acetic acid I...
08 September 2015 984 8 View
Hello, i am currently doing a research which involves the use of proteins,buffers and HPLC. I am planning on using acetate buffer for the entirety of the research. However, the researches i am...
08 September 2015 447 4 View
Could you recommend some articles on Urban Transportation System optimization and Innovation?
13 August 2024 2,595 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone What is your opinion about the introduction of an expert decision support system in which the rules are extracted from existing data without human intervention, instead of being...
31 July 2024 5,785 4 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View