How do you determine concentration of bound protein using Bradford Assay Method?

Hello, I am currently using LDH and bounding to toyo pearl chromatographic particles. I know I can determine the concentration of the protein in a buffer solution using the Bradford Assay Method....

11 December 2015 8,553 7 View

What is the diameter of LDH?

10 November 2015 952 0 View

How to determine protein concentration in a solution?

10 November 2015 3,456 0 View

How do we measure the concentration of immoblized LDH protein?

hello, I am currently using LDH protein and have so far made a mixture of the LDH solution (in a phosphate buffer) and toyopearl chromatograph particles. Now, I want to determine how much of the...

10 November 2015 1,475 3 View

How to determine how particles are inside in a PEEK column?

09 October 2015 5,539 2 View

How do to calculate dead volume?

Hello, I was wondering if any one knew how I can determine the dead volume in HPLC run. Is there any particular output I am looking for from the HPLC?

09 October 2015 6,408 3 View

What is a good buffer system to use for the enzyme LDH?

Is a acetate buffer a good buffer system to use for LDH? i know acetate buffer has a range of 3-6. is this a good enough buffer. if not what is a good buffer system for LDH?

08 September 2015 2,444 0 View

How do I prepare a 0.1M acetate buffer?

08 September 2015 855 8 View

Why treat proteins with different buffers?

Hello, i am currently doing a research which involves the use of proteins,buffers and HPLC. I am planning on using acetate buffer for the entirety of the research. However, the researches i am...

08 September 2015 394 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View