How do you determine concentration of bound protein using Bradford Assay Method?

Hello, I am currently using LDH and bounding to toyo pearl chromatographic particles. I know I can determine the concentration of the protein in a buffer solution using the Bradford Assay Method....

11 December 2015 8,553 7 View

What is the diameter of LDH?

10 November 2015 952 0 View

How to determine protein concentration in a solution?

10 November 2015 3,456 0 View

How do we measure the concentration of immoblized LDH protein?

hello, I am currently using LDH protein and have so far made a mixture of the LDH solution (in a phosphate buffer) and toyopearl chromatograph particles. Now, I want to determine how much of the...

10 November 2015 1,475 3 View

How to determine how particles are inside in a PEEK column?

09 October 2015 5,539 2 View

What is the Size of LDH protein?

09 October 2015 2,202 1 View

What is a good buffer system to use for the enzyme LDH?

Is a acetate buffer a good buffer system to use for LDH? i know acetate buffer has a range of 3-6. is this a good enough buffer. if not what is a good buffer system for LDH?

08 September 2015 2,444 0 View

How do I prepare a 0.1M acetate buffer?

08 September 2015 855 8 View

Why treat proteins with different buffers?

Hello, i am currently doing a research which involves the use of proteins,buffers and HPLC. I am planning on using acetate buffer for the entirety of the research. However, the researches i am...

08 September 2015 394 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View