Isaac Abraham

10 Questions 7 Answers 0 Followers

Questions related from Isaac Abraham

How do you determine concentration of bound protein using Bradford Assay Method?

Hello, I am currently using LDH and bounding to toyo pearl chromatographic particles. I know I can determine the concentration of the protein in a buffer solution using the Bradford Assay Method....

12 December 2015 8,672 7 View

How to determine protein concentration in a solution?

Hello, If i did reaction in order to attached a protein to a chromatographic particles and if i prepare a standard curve and determine the concentration and i further react the protein and...

11 November 2015 3,494 0 View

What is the diameter of LDH?

Hello Everyone, I was wondering if the diameter of LDH is determined in a research paper? if someone can point me to that research paper or web page, i would really appreciate it. Thank you.

11 November 2015 992 0 View

How do we measure the concentration of immoblized LDH protein?

hello, I am currently using LDH protein and have so far made a mixture of the LDH solution (in a phosphate buffer) and toyopearl chromatograph particles. Now, I want to determine how much of the...

11 November 2015 1,509 3 View

What is the Size of LDH protein?

I was wondering what the size of the LDH protein is?

10 October 2015 2,313 1 View

How do to calculate dead volume?

Hello, I was wondering if any one knew how I can determine the dead volume in HPLC run. Is there any particular output I am looking for from the HPLC?

10 October 2015 6,446 3 View

How to determine how particles are inside in a PEEK column?

Hello all, When packing a PEEK column, one that's is opaque. Is there a technique or method used to determine how much particles are packed inside? Is there a way to determine that? thank you

10 October 2015 5,580 2 View

What is a good buffer system to use for the enzyme LDH?

Is a acetate buffer a good buffer system to use for LDH? i know acetate buffer has a range of 3-6. is this a good enough buffer. if not what is a good buffer system for LDH?

09 September 2015 2,476 0 View

Why treat proteins with different buffers?

Hello, i am currently doing a research which involves the use of proteins,buffers and HPLC. I am planning on using acetate buffer for the entirety of the research. However, the researches i am...

09 September 2015 429 4 View

How do I prepare a 0.1M acetate buffer?

Hello, I am in the process of making mobile phases for my HPLC research and I had one question about buffer. The way I prepared my 0.1M acetate buffer was as follows. For the acetic acid I...

09 September 2015 965 8 View