I am trying to analyse cell cycle using propidium iodide staining. Can anyone tell me what the DNA content vs events curve would look like if the cells are not treated with RNase?
Hii,
The attached article has well explained your query (Cells are not treated with RNase).
Article Modified Annexin V/Propidium Iodide Apoptosis Assay For Accu...
cheers!!!
Dear Saurabh, Interesting article you enclosed, nice info,
Best,
Subhash
PI binds with both DNA and RNA, and hence RNase is very much essential to degrade the RNA.
Does it has to be RNase A, or it can be RNase H?
Is 5% CO2 incubation is necessary for RNAse treatment?
The question Irina asked is also my question: Does it has to be RNase A, or it can be RNase H?
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