Ayon Hassan

6 Questions 8 Answers 0 Followers

Questions related from Ayon Hassan

Has anyone detected immunoprecipitated endogenous protein by Coomasie or Silver Stain?

I am trying to detect an endogenous protein of around 135KDa by Coomassie or Silver Stain for a downstream analysis. My immunoprecipitation works well, and the protein seems highly enriched in...

13 January 2023 7,512 4 View

Does anyone have human primer sequences for making 28S ribosomal RNA probes for ISH?

I am trying to establish a control probe for RNA in situ hybridization for human samples. The plan is to synthesize DIG-labeled probes by in vitro transcription. Does anyone have primer sequences...

18 July 2022 5,305 0 View

Does anyone have any recommendation for a good kit for total RNA isolation from small sample size?

I am looking for a kit that can yield good quality RNA from small sample size (around 10 000 - 15 000 cells). I have tried with Trizol, but so far have been unsuccessful in getting good enough RNA...

23 May 2019 2,072 7 View

What happens if no RNase is added in PI staining?

I am trying to analyse cell cycle using propidium iodide staining. Can anyone tell me what the DNA content vs events curve would look like if the cells are not treated with RNase?

21 September 2018 6,548 6 View

Can anyone help me with TIDE (CRISPR/Cas9)?

I transfected Cas9 expressing cells with two sgRNAs targeting two different genes (separately, not co-transfection). Both proteins show significant Knockdown in Western Blot, indicating that the...

03 July 2018 8,448 4 View

Do CRISPR/Cas9 monoallelic knockout clones eventually become biallelic knockout?

I have a conceptual question. It is a commonly observed phenomenon when using the CRISPR/Cas9 system that edited cells harbor monoallelic knockout. However, in cells stably expressing Cas9 and...

28 May 2018 435 7 View