Please take this survey?

Hello! I am a second year Clinical Psychology student at William James College. I have conducted a survey as part of my Doctoral requirement that measures the effectiveness of Cognitive Load...

12 February 2020 2,709 3 View

Metabolites (antibiotic) extraction from microbes?

Can we centrifuge culture broth that already mix with solvent? Or we must centrifuge first to collect supernatant and add the solvent to supernatant?

05 June 2019 923 3 View

Extracting metabolites from actinomycetes?

02 March 2019 9,275 3 View

Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

24 January 2019 5,731 1 View

Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

31 December 2018 9,281 2 View

What is in the supernatant after centrifugation?

What happen If we add methanol to supernatant? Will they form two layers ? (Organic and aqueous phase) Or just one phase? Why we use methanol instead water for extraction? (antibiotic compound)

10 November 2018 8,295 4 View

Why are my extracts (glucosamine) not completely soluble in water?

Hi, I have extracted glucosamine from lobsters shell, however my extracts are not soluble in water,Could you please tell me why and give me a suitable way to dissolve them in water? Thank you

04 May 2018 7,295 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

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02 March 2021 3,060 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View