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Questions related from Ervina ..
Can we centrifuge culture broth that already mix with solvent? Or we must centrifuge first to collect supernatant and add the solvent to supernatant?
06 June 2019 968 3 View
extract metabolites from actinomycetes usually, culture broth was harvested by centrifugation at 4 000 r/min for 15 min. The supernatant was collected and added equal volumes of solvent. does...
03 March 2019 9,317 3 View
If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?
01 January 2019 9,345 2 View
Will they form two layers ? (Organic and aqueous phase)?
11 November 2018 1,574 1 View
What happen If we add methanol to supernatant? Will they form two layers ? (Organic and aqueous phase) Or just one phase? Why we use methanol instead water for extraction? (antibiotic compound)
11 November 2018 8,402 4 View
Hi, I have extracted glucosamine from lobsters shell, however my extracts are not soluble in water,Could you please tell me why and give me a suitable way to dissolve them in water? Thank you
05 May 2018 7,349 3 View